Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)
![Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)](https://www.abcam.com/ps/products/280/ab280902/Images/ab280902-459238-anti-tie2-antibody-33-western-blot-huvec.jpg "Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [33] to TIE2 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TIE2 antibody [33] - BSA and Azide free
See all TIE2 primary antibodies -
Description
Mouse monoclonal [33] to TIE2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC lysates. Flow Cyt: HUVEC cells.
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General notes
ab280902 is the carrier-free version of ab280905. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280902 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
33 -
Isotype
IgG1 -
Light chain type
kappa
Images
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Western blot - Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)All lanes : Anti-TIE2 antibody [33] (ab280905) at 1/1000 dilution
Lane 1 : HUVEC(human umbilical vein endothelial cell ), whole cell lysate
Lane 2 : 293T ( human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 125 kDa
Observed band size: 120,150 kDa why is the actual band size different from the predicted?This data was developed using ab280905, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:Â 28396397)
Negative control: HEK-293T (PMID: 17189382).
Exposure time: 37 seconds
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![Flow Cytometry - Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)](https://www.abcam.com/ps/products/280/ab280902/Images/ab280902-1-anti-tie2-antibody-33-flow-cytometry-hek293.jpg)
Flow Cytometry - Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)This data was developed using ab280905 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HEK-293 (Human embryonic kidney epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling TIE2 with ab280905 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293.
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![Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)](https://www.abcam.com/ps/products/280/ab280902/Images/ab280902-2-benefitsofrecombinantantibodies.jpg)
Anti-TIE2 antibody [33] - BSA and Azide free (ab280902)
