Anti-Thyroxine Binding Globulin antibody (ab48646)
Key features and details
- Rabbit polyclonal to Thyroxine Binding Globulin
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Thyroxine Binding Globulin antibody
See all Thyroxine Binding Globulin primary antibodies -
Description
Rabbit polyclonal to Thyroxine Binding Globulin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Recombinant fragment
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Immunogen
Human TBG purified from human plasma
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Positive control
- ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Thyroxine Binding Globulin antibody (ab48646)ICC/IF image of ab48646 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48646, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Thyroxine Binding Globulin antibody (ab48646)Ab48646 recognizes the full length recombinant thyroxine binding globulin protein which has an expected molecular weight of 55 kDa due to glycosylation.
