Anti-Thymidylate Synthase antibody (ab7398)
Key features and details
- Sheep polyclonal to Thymidylate Synthase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thymidylate Synthase antibody
See all Thymidylate Synthase primary antibodies -
Description
Sheep polyclonal to Thymidylate Synthase -
Host species
Sheep -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
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General notes
The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.