Anti-Thymidine Kinase 1/TK1 antibody (ab75277)
Key features and details
- Rabbit polyclonal to Thymidine Kinase 1/TK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thymidine Kinase 1/TK1 antibody
See all Thymidine Kinase 1/TK1 primary antibodies -
Description
Rabbit polyclonal to Thymidine Kinase 1/TK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Thymidine Kinase 1/TK1.
Database link: NP_003249.1 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
This product was previously labelled as Thymidine Kinase 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Thymidine Kinase 1/TK1 antibody (ab75277)All lanes : Anti-Thymidine Kinase 1/TK1 antibody (ab75277) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 130 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Thymidine Kinase 1/TK1 antibody (ab75277)Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
