Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15225] to Thioredoxin 2
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Thioredoxin 2 antibody [EPR15225]
    See all Thioredoxin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15225] to Thioredoxin 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Raji and HeLa cell lysate. Human liver and mouse kidney tissue. HeLa and K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    All lanes : Anti-Thioredoxin 2 antibody [EPR15225] (ab185544) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Thioredoxin 2 with ab185544 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Flow Cytometry - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Flow Cytometry - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Flow cytometry analysis of 2% paraformaldehyde fixed K562 cells labeling Thioredoxin 2 with ab185544 at 1/150 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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