Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TFPI2 antibody [EPR14442] (ab186747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14442] to TFPI2
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TFPI2 antibody [EPR14442]
    See all TFPI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14442] to TFPI2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue sections. WB: Jurkat, MCF7, rat spleen, HeLa, mouse heart, mouse brain, mouse kidney and mouse spleen lysates. IP: TFPI2 IP in HeLa cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
    Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
    All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
    Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
    All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/10000 dilution

    Lane 1 : Rat spleen lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
    Western blot - Anti-TFPI2 antibody [EPR14442] (ab186747)
    All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (ab186747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] (ab186747)

    Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (ab186747)
    Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] (ab186747)

    Lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer: 5% NFDM/TBST.

  • Anti-TFPI2 antibody [EPR14442] (ab186747)
    Anti-TFPI2 antibody [EPR14442] (ab186747)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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