Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-TFF2 antibody [EPR23231-208] (ab267474)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23231-208] to TFF2
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-TFF2 antibody [EPR23231-208]
See all TFF2 primary antibodies
Description

Rabbit monoclonal [EPR23231-208] to TFF2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human stomach lysate. IHC-P: Human stomach tissue. IP: Human stomach lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23231-208
Isotype

IgG
Research areas

Western blot - Anti-TFF2 antibody [EPR23231-208] (ab267474)

All lanes : Anti-TFF2 antibody [EPR23231-208] (ab267474) at 1/1000 dilution

Lane 1 : Human stomach tissue lysate
Lane 2 : Human heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

TFF2 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:10716671)

Negative Control: Human heart (PMID:10452549)

Exposure time: 37 seconds
Immunoprecipitation - Anti-TFF2 antibody [EPR23231-208] (ab267474)

TFF2 was immunoprecipitated from 0.35 mg Human stomach lysate with ab267474 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267474 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Human stomach lysate 10ug

Lane 2: ab267474 IP in Human stomach lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267474 in Human stomach lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds

TFF2 is a glycoprotein. The 24 kDa band is likely to be a higher glycosylated TFF2. (PMID:10716671)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (ab267474)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TFF2 with ab267474 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human lung (PMID: 17242463). The section was incubated with ab267474 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (ab267474)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TFF2 with ab267474 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human stomach (PMID: 17242463) The section was incubated with ab267474 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-TFF2 antibody [EPR23231-208] (ab267474)

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