Anti-TFF2 antibody [EPR23231-208] (ab267474)
![Anti-TFF2 antibody [EPR23231-208] (ab267474)](https://www.abcam.com/ps/products/267/ab267474/Images/ab267474-370147-267474WB1anti-tff2-antibody-epr23231-208-western-blot-human.jpg "Anti-TFF2 antibody [EPR23231-208] (ab267474)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23231-208] to TFF2
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-TFF2 antibody [EPR23231-208]
See all TFF2 primary antibodies -
Description
Rabbit monoclonal [EPR23231-208] to TFF2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human stomach lysate. IHC-P: Human stomach tissue. IP: Human stomach lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23231-208 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TFF2 antibody [EPR23231-208] (ab267474)All lanes : Anti-TFF2 antibody [EPR23231-208] (ab267474) at 1/1000 dilution
Lane 1 : Human stomach tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
TFF2 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:10716671)
Negative Control: Human heart (PMID:10452549)
Exposure time: 37 seconds
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![Immunoprecipitation - Anti-TFF2 antibody [EPR23231-208] (ab267474)](https://www.abcam.com/ps/products/267/ab267474/Images/ab267474-3-267474IP1anti-tff2-antibody-epr23231-208-immunoprecipitation-stomach-human.jpg)
Immunoprecipitation - Anti-TFF2 antibody [EPR23231-208] (ab267474)TFF2 was immunoprecipitated from 0.35 mg Human stomach lysate with ab267474 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267474 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Human stomach lysate 10ug
Lane 2: ab267474 IP in Human stomach lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267474 in Human stomach lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
TFF2 is a glycoprotein. The 24 kDa band is likely to be a higher glycosylated TFF2. (PMID:10716671)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (ab267474)](https://www.abcam.com/ps/products/267/ab267474/Images/ab267474-2-267474IHC2anti-tff2-antibody-epr23231-208-immunohistochemistry-lung-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (ab267474)Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TFF2 with ab267474 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human lung (PMID: 17242463). The section was incubated with ab267474 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (ab267474)](https://www.abcam.com/ps/products/267/ab267474/Images/ab267474-1-267474IHC1anti-tff2-antibody-epr23231-208-immunohistochemistry-stomach-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (ab267474)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TFF2 with ab267474 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human stomach (PMID: 17242463) The section was incubated with ab267474 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-TFF2 antibody [EPR23231-208] (ab267474)](https://www.abcam.com/ps/products/267/ab267474/Images/ab267474-5-benefits-of-recombinant-antibodies.png)
Anti-TFF2 antibody [EPR23231-208] (ab267474)
