Anti-TDP1 antibody (ab4166)
Key features and details
- Rabbit polyclonal to TDP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-TDP1 antibody
See all TDP1 primary antibodies -
Description
Rabbit polyclonal to TDP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Human TDP1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13764)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanRecombinant fragmentAll applications MouseRatApplication Abreviews Notes WB (3) Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).Abcam recommends blocking with 1-3% Milk.
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).Abcam recommends blocking with 1-3% Milk.
Target
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Function
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. -
Tissue specificity
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. -
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy -
Sequence similarities
Belongs to the tyrosyl-DNA phosphodiesterase family. -
Post-translational
modificationsPhosphorylated on serine and/or threonine residues, but not on tyrosine residues. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55775 Human
- Entrez Gene: 104884 Mouse
- Entrez Gene: 314380 Rat
- Omim: 607198 Human
- SwissProt: Q9NUW8 Human
- SwissProt: Q8BJ37 Mouse
- SwissProt: Q4G056 Rat
- Unigene: 209945 Human
see all -
Alternative names
- AI838772 antibody
- AW493413 antibody
- FLJ11090 antibody
see all
Images
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Western blot - Anti-TDP1 antibody (ab4166)Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Western blot - Anti-TDP1 antibody (ab4166)This image is courtesy of Keith Caldecott, University of SussexLane 1 : Molecular weight markers
Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution
Lane 2 : 1 ng purified recombinant human TDP1
Lane 3 : 3 ng purified recombinant human TDP1
Lane 4 : 6 ng purified recombinant human TDP1
Lane 5 : 6 micrograms total protein from MCF7 human cell extract
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaab4166 was incubated for 1 hr at room temp.
Protocols
Datasheets and documents
References (22)
ab4166 has been referenced in 22 publications.
- Ghosh A et al. SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy. Sci Adv 5:eaax9778 (2019). PubMed: 31723605
- Rehman I et al. PRMT5-mediated arginine methylation of TDP1 for the repair of topoisomerase I covalent complexes. Nucleic Acids Res 46:5601-5617 (2018). PubMed: 29718323
- Meisenberg C et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res 45:1159-1176 (2017). WB ; Human . PubMed: 28180300
- Chiang SC et al. Mitochondrial protein-linked DNA breaks perturb mitochondrial gene transcription and trigger free radical-induced DNA damage. Sci Adv 3:e1602506 (2017). PubMed: 28508041
- Takiuchi Y et al. HTLV-1 bZIP factor suppresses TDP1 expression through inhibition of NRF-1 in adult T-cell leukemia. Sci Rep 7:12849 (2017). PubMed: 28993637
Images
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Western blot - Anti-TDP1 antibody (ab4166)Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
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Western blot - Anti-TDP1 antibody (ab4166) This image is courtesy of Keith Caldecott, University of SussexLane 1 : Molecular weight markers
Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution
Lane 2 : 1 ng purified recombinant human TDP1
Lane 3 : 3 ng purified recombinant human TDP1
Lane 4 : 6 ng purified recombinant human TDP1
Lane 5 : 6 micrograms total protein from MCF7 human cell extract
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaab4166 was incubated for 1 hr at room temp.
