Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)
![Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)](https://www.abcam.com/ps/products/80/ab80579/Images/ab80579-392213-antitautau55ugPFAhuipsc.jpg "Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)")
Key features and details
- Mouse monoclonal [TAU-5] to Tau - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Tau antibody [TAU-5] - BSA and Azide free
See all Tau primary antibodies -
Description
Mouse monoclonal [TAU-5] to Tau - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseRatHuman
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Immunogen
Full length native protein (purified) corresponding to Cow Tau. Purified bovine microtubule-associated proteins.
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Positive control
- WB: Human Alzheimer's brain whole tissue lysate. Human, mouse and rat brain whole tissue lysate. ICC: Human iPSC-Derived Glutamatergic Neurons. SKNSH cells treated with prostaglandin J2.
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General notes
This antibody is without BSA and sodium azide.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TAU-5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)
Ab80579 staining Tau in ab259259 ioNEURONS/glut cells (Human iPSC-Derived Glutamatergic Neurons).
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab80579 at 5 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution (shown in green) and ab150088, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) preabsorbed at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Images were acquired with the Perkin Elmer Operetta HCA and a single confocal plane is shown.
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![Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)](https://www.abcam.com/ps/products/80/ab80579/Images/ab80579-333426-ab80579AP4408749MILKSHEATAUBRACKETs.jpg)
Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)All lanes :
Lane 1 : Human Alzheimer's brain whole tissue lysate
Lane 2 : Human brain whole tissue lysate
Lane 3 : Mouse brain whole tissue lysate
Lane 4 : Rat brain whole tissue lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 79 kDaTau cleavage products are shown between 70kDa and 50kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before ab80579 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)](https://www.abcam.com/ps/products/80/ab80579/Images/ab80579-207976-anti-tau-antibody-tau-5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)ab80579 staining tau in SKNSH cells treated with prostaglandin J2 (ab120913), by ICC/IF. Expression of tau expression is restringed to the perinuclear zone with increased concentration of prostaglandin J2, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120913 (prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab80579 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).
