Anti-TAF1 antibody (ab188427)
Key features and details
- Rabbit polyclonal to TAF1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TAF1 antibody
See all TAF1 primary antibodies -
Description
Rabbit polyclonal to TAF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanSyrian hamsterWB Human
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Immunogen
Synthetic peptide corresponding to Human TAF1 aa 100-200 conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: P21675 -
Positive control
- HeLa whole cell extract; BHK and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TAF1 antibody (ab188427)
Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.
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Western blot - Anti-TAF1 antibody (ab188427)Anti-TAF1 antibody (ab188427) at 1/1000 dilution + HeLa whole cell extract at 10 µg
Predicted band size: 213 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-TAF1 antibody (ab188427)IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.
Panel a): Stained with ab188427 without immunizing peptide.
Panel b): Stained with ab188427 with immunizing peptide.
Panel c): Panel a) co-stained with Hoechst 33258.
Panel d): Panel b) co-stained with Hoechst 33258.
Panel e): Phase-contrast image of panels a) and c).
Panel f): Phase-contrast image of panels b) and d).
