Anti-SV2A antibody (ab77177)
Key features and details
- Goat polyclonal to SV2A
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SV2A antibody
See all SV2A primary antibodies -
Description
Goat polyclonal to SV2A -
Host species
Goat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Horse, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey
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Immunogen
Synthetic peptide:
C-TVELYPSDKRTTA
, corresponding to internal sequence amino acids 673-685 of Human SV2A (NP_055664.2) -
Positive control
- WB: Rat brain tissue lysate. ICC: U2OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77177 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-SV2A antibody (ab77177)
Immunofluorescence analysis of U2OS cells labelling SV2A with ab77177 at 10 µg/mL. Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488 secondary antibody (2 µg/mL) was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Western blot - Anti-SV2A antibody (ab77177)All lanes : Anti-SV2A antibody (ab77177) at 0.3 µg/ml
Lane 1 : Rat brain lysate in RIPA buffer
Lane 2 : Rat brain lysate in RIPA buffer treated with immunising peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Primary antibody incubation: 1 hour at room temperature.
An additonal band of unknown identity was also consistently observed at 28kDa, which was successfully blocked by incubation with the immunising peptide.
