Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21774-153] to Surfactant protein D/SP-D
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Recombinant fragment

Overview

  • Product name

    Anti-Surfactant protein D/SP-D antibody [EPR21774-153]
    See all Surfactant protein D/SP-D primary antibodies

  • Description

    Rabbit monoclonal [EPR21774-153] to Surfactant protein D/SP-D

  • Host species

    Rabbit

  • Specificity

    The Mouse species recommendation is based on the WB results. We do not guarantee IHC for Mouse.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    WB
    Mouse
    Rat
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse Surfactant protein D/SP-D recombinant protein (aa 1-374); Mouse lung tissue lysate; Rat lung tissue lysate. IHC-P: Rat lung tissue. IHC-Fr: Rat lung tissue.

  • General notes

    Previously labelled as Surfactant protein D.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422) at 1/1000 dilution + Mouse Surfactant protein D/SP-D recombinant protein (aa 1-374) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Recombinant protein was obtained from a mammalian expression system.

  • Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Western blot - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    All lanes : Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Rat lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 8 seconds.

    Lane 2: 26 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 9751757, PMID: 23390139).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Surfactant protein D/SP-D with ab220422 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in Clara cells and alveolar type II cells of rat lung (PMID: 10820266) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using citrate buffer, pH 6.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

    Negative control: no staining on rat skeletal muscle (PMID: 10640760).

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Surfactant protein D/SP-D with ab220422 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval using citrate buffer, pH 6.0.

  • Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Immunohistochemistry (Frozen sections) - Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

    Immunohistochemical analysis of frozen sectioned 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat lung tissue labeling Surfactant protein D/SP-D with ab220422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in Clara cells and alveolar type II cells of rat lung (PMID: 2049389) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (pH 9.0).

  • Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)
    Anti-Surfactant protein D/SP-D antibody [EPR21774-153] (ab220422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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