Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4602] to Sumo 2 + Sumo 3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free
    See all Sumo 2 + Sumo 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4602] to Sumo 2 + Sumo 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247742 is the carrier-free version of ab109005.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4602

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    This data was developed using ab109005, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 4 : SW480 cell lysate
    Lane 5 : HL60 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 11 kDa



    This data was developed using ab109005, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    This data was developed using ab109005, the same antibody clone in a different buffer formulation.ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    This data was developed using ab109005, the same antibody clone in a different buffer formulation.ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution

    Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
    Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
    Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
    Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab109005, the same antibody clone in a different buffer formulation.
  • Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)
    Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free (ab247742)

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