Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539)
Key features and details
- Rabbit polyclonal to Streptolysin O (Hemolytic streptococcus)
- Suitable for: WB, Neutralising, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Streptolysin O (Hemolytic streptococcus) antibody -
Description
Rabbit polyclonal to Streptolysin O (Hemolytic streptococcus) -
Host species
Rabbit -
Specificity
ab188539 reacts with Streptolysin O of Group A, C and G origins. -
Tested applications
Suitable for: WB, Neutralising, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein (His-tag) corresponding to Streptolysin O (Hemolytic streptococcus). (from S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539)All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution
Lane 1 : Medium only
Lane 2 : Culture supernatant of GAS strain Sa
Lane 3 : Culture supernatant of GCS strain H46A
Predicted band size: 64 kDaMost of Streptolysin O in culture medium of GAS strain Sa is proteolytically degraded, accounting for low activity level of Streptolysin O in the culture of this strain. Condensed sample is analyzed in the second Western Blot.
The two faint bands below Streptolysin O band are partially degraded Streptolysin O.
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Western blot - Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539)All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution
Lane 1 : Concentrated culture of GSA strain Sa
Lane 2 : Concentrated medium only
Predicted band size: 64 kDaCulture supernatant was approximately 1,000~2,000 fold concentrated from culture medium by ammonium fractionation and heat-precipitation.
The Streptolysin O of GSA strains is degraded by an exoprotease called streptococcal pyrogenic exotoxin B and both the concentration and size of Streptolysin O in culture medium is decreased from the native one.
