Anti-STAT6 antibody [21HCLC] (ab277765)
![Anti-STAT6 antibody [21HCLC] (ab277765)](https://www.abcam.com/ps/products/277/ab277765/Images/ab277765-1-antiSTAT6recombinantpolyclonalantibody21hclcChIP.jpg "Anti-STAT6 antibody [21HCLC] (ab277765)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [21HCLC] to STAT6
- Suitable for: ChIP, WB
- Reacts with: Human
Overview
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Product name
Anti-STAT6 antibody [21HCLC]
See all STAT6 primary antibodies -
Description
Rabbit recombinant multiclonal [21HCLC] to STAT6 -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human STAT6 aa 600-700.
Database link: P42226 -
Positive control
- ChIP: HeLa cells treated with IFN-gamma. WB: HeLa, A431, A549 whole cell lysate, HeLa cells treated with IFN1a.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
21HCLC -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-STAT6 antibody (ab277765)
ChIP- qPCR analysis of STAT6 was performed with 3 μg/mL of ab277765 on sheared chromatin from 2 million HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with IFN-gamma (50 ng/mL) for 1h using the MAGnify Chromatin Immunoprecipitation System. Normal rabbit IgG (3 μg/mL) was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus Real-Time PCR System with primers for the promoter of active AP-1 and COX2 gene, used as positive control targets, and the inactive SAT2 satellite repeat, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
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Western blot - Anti-STAT6 antibody (ab277765)All lanes : Anti-STAT6 antibody [21HCLC] (ab277765) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG-HRP at 1/5000 dilution
Predicted band size: 94 kDa
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Western blot - Anti-STAT6 antibody (ab277765)Anti-STAT6 antibody [21HCLC] (ab277765) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with IFN1a (150 ng/mL, 15 min)
Predicted band size: 94 kDa
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![Anti-STAT6 antibody [21HCLC] (ab277765)](https://www.abcam.com/ps/products/277/ab277765/Images/ab277765-7-benefits-of-recombinant-antibodies.jpg)
Anti-STAT6 antibody [21HCLC] (ab277765)