Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT2 antibody [22/Stat2] (ab280900)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [22/Stat2] to STAT2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT2 antibody [22/Stat2]
    See all STAT2 primary antibodies

  • Description

    Mouse monoclonal [22/Stat2] to STAT2

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type HeLa whole cell lysate. Ramos, K-562 and THP1 whole cell lysate. IP: Ramos whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)
    Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)
    All lanes : Anti-STAT2 antibody [22/Stat2] (ab280900) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
    Lane 2 : STAT2 knockout HeLa whole cell lysate
    Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97,113 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    Lanes 1 - 3: Merged signal (red and green). Green - ab280900 observed at 97, 113 kDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab280900 Anti-Stat2 antibody was shown to react with STAT2 in HeLa cells in Western blot. Loss of signal was observed when STAT2 knockout sample was used. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE.

    ab280900 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-STAT2 antibody [22/Stat2] (ab280900)
    Immunoprecipitation - Anti-STAT2 antibody [22/Stat2] (ab280900)

    Stat2 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 µg with ab280900 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab280900 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

    Lane 1: Ramos whole cell lysate 10 µg

    Lane 2: ab280900 IP in Ramos whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (ab18413) instead of ab280900 in Ramos whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)
    Western blot - Anti-STAT2 antibody [22/Stat2] (ab280900)
    All lanes : Anti-STAT2 antibody [22/Stat2] (ab280900) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 2 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
    Lane 3 : THP1 (human monocytic leukemia monocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 97 kDa
    Observed band size: 97,113 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 92 seconds

  • Anti-STAT2 antibody [22/Stat2] (ab280900)
    Anti-STAT2 antibody [22/Stat2] (ab280900)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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