Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10006(2)] to ST3GAL3
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-ST3GAL3 antibody [EPR10006(2)]
See all ST3GAL3 primary antibodies
Description

Rabbit monoclonal [EPR10006(2)] to ST3GAL3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human ST3GAL3 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q11203
Positive control
- Fetal heart and human skeletal muscle lysates; fetal cardiac muscle tissue; Human skeletal muscle tissue; A673 cells; permealized Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10006(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

Immunohistochemical analysis of Human skeletal muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

Flow cytometric analysis of permeabilized Jurkat cells labeling ST3GAL3 using ab177959 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

Immunofluorescence analysis of A673 cells labeling ST3GAL3 using ab177959 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

Immunohistochemical analysis of fetal cardiac muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

All lanes : Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 42 kDa
Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com