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Anti-SSX2IP antibody [EPR16979] (ab199425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16979] to SSX2IP
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SSX2IP antibody [EPR16979]
    See all SSX2IP primary antibodies

  • Description

    Rabbit monoclonal [EPR16979] to SSX2IP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK293 and Jurkat whole cell lysates. Human testis lysates. IHC-P: Human cerebral cortex and human kidney tissue. IP: HEK293 whole cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (ab199425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (ab199425)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425)
    Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425)
    All lanes : Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68, 71 kDa
    Observed band size: 68, 71 kDa


    Exposure time: 1 minute


    Based on the sequence analysis, ab199425 recognizes two isoforms with the predicted MWs of 71KDa, and 68KDa, respectively.

  • Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (ab199425)
    Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (ab199425)

    SSX2IP was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199425 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab199425 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Input HEK293 whole cell lysate (10 µg). Lane 2: HEK293 whole cell lysate folowing precipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199425 in HEK293 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.

  • Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425)
    Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425)
    Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/20000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 68, 71 kDa
    Observed band size: 68, 71 kDa


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (ab199425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (ab199425)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-SSX2IP antibody [EPR16979] (ab199425)
    Anti-SSX2IP antibody [EPR16979] (ab199425)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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