Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-SSX2IP antibody [EPR16979] (ab199425)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16979] to SSX2IP
Suitable for: IP, IHC-P, WB
Reacts with: Human

Product name

Anti-SSX2IP antibody [EPR16979]
See all SSX2IP primary antibodies
Description

Rabbit monoclonal [EPR16979] to SSX2IP
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK293 and Jurkat whole cell lysates. Human testis lysates. IHC-P: Human cerebral cortex and human kidney tissue. IP: HEK293 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16979
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (ab199425)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425)

All lanes : Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68, 71 kDa
Observed band size: 68, 71 kDa

Exposure time: 1 minute

Based on the sequence analysis, ab199425 recognizes two isoforms with the predicted MWs of 71KDa, and 68KDa, respectively.
Immunoprecipitation - Anti-SSX2IP antibody [EPR16979] (ab199425)

SSX2IP was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199425 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab199425 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Input HEK293 whole cell lysate (10 µg). Lane 2: HEK293 whole cell lysate folowing precipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199425 in HEK293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.
Western blot - Anti-SSX2IP antibody [EPR16979] (ab199425)

Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/20000 dilution + Human testis lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 68, 71 kDa
Observed band size: 68, 71 kDa

Exposure time: 30 seconds

Blocking/dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSX2IP antibody [EPR16979] (ab199425)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-SSX2IP antibody [EPR16979] (ab199425)

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