Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SSRP1 antibody [10D7] (ab26212)

Key features and details

  • Mouse monoclonal [10D7] to SSRP1
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-SSRP1 antibody [10D7]
    See all SSRP1 primary antibodies

  • Description

    Mouse monoclonal [10D7] to SSRP1

  • Host species

    Mouse

  • Specificity

    ab26212 recognises Structure specific recognition protein 1 (SSRP1).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human SSRP1.

  • Positive control

    • HeLa cell lysate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SSRP1 antibody [10D7] (ab26212)
    Western blot - Anti-SSRP1 antibody [10D7] (ab26212)
    Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate

    Secondary
    Goat anti-mouse Ig conjugated to HRP.

    Developed using the ECL technique.

    Predicted band size: 81 kDa
    Observed band size: 81 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)
    Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)
    ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)
    Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)
    Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

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