Anti-SS18 antibody [EPR23636-98] (ab254405)
![Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-447670-anti-ss18-antibody-epr2363698-western-blot-adenocarcinoma.jpg "Anti-SS18 antibody [EPR23636-98] (ab254405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23636-98] to SS18
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-SS18 antibody [EPR23636-98]
See all SS18 primary antibodies -
Description
Rabbit monoclonal [EPR23636-98] to SS18 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanIP HumanWB MouseRatHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, HEK-293T, NIH/3T3, C6 and Neuro-2a whole cell lysates. IHC-P: Human cervical carcinoma and synovial sarcoma tissue; Mouse colon tissue; rat cardiac muscle tissue. IP: HeLa whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23636-98 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405)All lanes : Anti-SS18 antibody [EPR23636-98] (ab254405) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 45,55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:23540691;29374058)
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure times: Lanes 1-3: 59 seconds; Lane 4: 3 minutes; Lane 5: 59 seconds.
-
![Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-5-anti-ss18-antibody-epr2363698-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (ab254405)SS18 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab254405 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254405 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab254405 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254405 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-1-anti-ss18-antibody-epr2363698-immunohistochemistry-human-cervical-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human cervical carcinoma. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-2-anti-ss18-antibody-epr2363698-immunohistochemistry-human-synovial-sarcoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)Immunohistochemical analysis of paraffin-embedded Human synovial sarcoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in Human synovial sarcoma (PMID: 27120803). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-3-anti-ss18-antibody-epr2363698-immunohistochemistry-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse colon. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-4-anti-ss18-antibody-epr2363698-immunohistochemistry-rat-cardiac-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat cardiac muscle. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-SS18 antibody [EPR23636-98] (ab254405)](https://www.abcam.com/ps/products/254/ab254405/Images/ab254405-448068-benefitsofrecombinantantibodies.png)
Anti-SS18 antibody [EPR23636-98] (ab254405)