Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Wednesday March 10, 2021

Anti-SS18 antibody [EPR23636-98] (ab254405)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23636-98] to SS18
Suitable for: IP, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-SS18 antibody [EPR23636-98]
See all SS18 primary antibodies
Description

Rabbit monoclonal [EPR23636-98] to SS18
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293T, NIH/3T3, C6 and Neuro-2a whole cell lysates. IHC-P: Human cervical carcinoma and synovial sarcoma tissue; Mouse colon tissue; rat cardiac muscle tissue. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23636-98
Isotype

IgG
Research areas

Western blot - Anti-SS18 antibody [EPR23636-98] (ab254405)

All lanes : Anti-SS18 antibody [EPR23636-98] (ab254405) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 45,55 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:23540691;29374058)

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

Exposure times: Lanes 1-3: 59 seconds; Lane 4: 3 minutes; Lane 5: 59 seconds.
Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] (ab254405)

SS18 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab254405 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254405 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2: ab254405 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254405 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 6 seconds.

Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human cervical carcinoma. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

Immunohistochemical analysis of paraffin-embedded Human synovial sarcoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in Human synovial sarcoma (PMID: 27120803). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse colon. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] (ab254405)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat cardiac muscle. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-SS18 antibody [EPR23636-98] (ab254405)

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