Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8760(2)] to SRGAP1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free
    See all SRGAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8760(2)] to SRGAP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249658 is the carrier-free version of ab171938.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    All lanes : Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : Human fetal lung tissue lysate
    Lane 5 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 124 kDa



    This data was developed using ab171938, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    This data was developed using ab171938, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293T cells labeling SRGAP1 with ab171938 at 1/100 (red) or a rabbit IgG (negative) (green).
  • Immunocytochemistry - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    Immunocytochemistry - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    This data was developed using ab171938, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    This data was developed using ab171938, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    This data was developed using ab171938, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
    Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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