Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)
![Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)](https://www.abcam.com/ps/products/269/ab269577/Images/ab269577-368353-AntiSREBP1-antibody-2A4-BSA-Azide-free-western-blot-hela-hap1-human.jpg "Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)")
Key features and details
- Mouse monoclonal [2A4] to SREBP1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-SREBP1 antibody [2A4] - BSA and Azide free
See all SREBP1 primary antibodies -
Description
Mouse monoclonal [2A4] to SREBP1 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Protein Array Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Epitope
Amino acids 301 - 407. -
Positive control
- WB: HeLa and HAP1 whole cell lysates.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269577 is the carrier-free version of ab3259. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
2A4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Images
-
Western blot - Anti-SREBP1 antibody [2A4] - BSA and Azide free (ab269577)All lanes : Anti-SREBP1 antibody [2A4] (ab3259) at 5 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 127 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This image was produced using the same antibody clone but in a different formulation ab3259, PBS and sodium azide.
