Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4374(3)] to SRC3 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free
    See all SRC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4374(3)] to SRC3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICCmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Daudi, HeLa and K562 cell lysates; Human endometrium adenocarcinoma tissue

  • General notes

    ab243600 is the carrier-free version of ab133611 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab243600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    Western blot - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    All lanes : Anti-SRC3 antibody [EPR4374(3)] (ab133611) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 155 kDa



    This data was developed using ab133611, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    This data was developed using ab133611, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab133611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133611, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    This data was developed using the same antibody clone in a different buffer formulation (ab133611 ).
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling SRC3 with purified ab133611 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.29 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    This data was developed using ab133611, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of KAT13B / AIB1 in paraffin embedded Human endometrium adenocarcinoma tissue, using ab133611 at a dilution of 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
    Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)

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