Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
![Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)](https://www.abcam.com/ps/products/2/ab2782/Images/ab2782-219026-anti-src3-antibody-ax153-chip-grade-immunohistochemistry.jpg "Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)")
Key features and details
- Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SRC3 antibody [AX15.3] - ChIP Grade
See all SRC3 primary antibodies -
Description
Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human SRC3 aa 605-1294.
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General notes
Locus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
AX15.3 -
Isotype
IgG1 -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting
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![Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)](https://www.abcam.com/ps/products/2/ab2782/Images/ab2782_1.jpg)
Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.
