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Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20451] to SQSTM1 / p62 (phospho S349) - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free
    See all SQSTM1 / p62 primary antibodies

  • Description

    Rabbit monoclonal [EPR20451] to SQSTM1 / p62 (phospho S349) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells treated with 2µM MG-132 for 18 hours.

  • General notes

    Ab238445 is the carrier-free version of ab211324. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238445 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)
    Flow Cytometry - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, treated with 2μM MG-132 for 18 hours (red) or untreated (green), labeling SQSTM1 / p62 (phospho S349) with ab211324 at 1/500 dilution compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211324).

  • Immunoprecipitation - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)
    Immunoprecipitation - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)

    SQSTM1 / p62 (phospho S349) was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 2μM MG-132 for 18h with ab211324 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab211324 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa treated with 2μM MG-132 for 18h whole cell lysate, 10 µg (Input).

    Lane 2: ab211324 IP in HeLa treated with 2μM MG-132 for 18h whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211324 in HeLa treated with 2μM MG-132 for 18h whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211324).

  • Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)
    Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, treated with 2μM MG-132 for 18 hours or untreated, labeling SQSTM1 / p62 (phospho S349) with ab211324 at 1/100 dilution, followed by Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (green).

    Confocal image showing cytoplasmic staining on HeLa cell line. The expression increased after treatment with 2μM MG-132 for 18 hours.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211324).

  • Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)
    Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free (ab238445)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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