Anti-SPOCD1 antibody (ab122188)
Key features and details
- Rabbit polyclonal to SPOCD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPOCD1 antibody -
Description
Rabbit polyclonal to SPOCD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 995-1085 (SPLLSPGLEV THSSLLLAVL LPKEGLPDTA GSSPWLGKVQ KMVSFNSKVE KRYYQPDDRR PNVPLKGTPP PGGAWQQSQG RGSIAPRGIS A) of Human SPOCD1.
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Positive control
- IHC-P: Human testis tissue; WB: U-251 MG and Human Plasma lysates; ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-SPOCD1 antibody (ab122188)Immunocytochemistry/immunofluorescence analysis of A431 cells labelling SPOCD1 with ab122188 shows positive staining in the nucleus and nucleoli. -
Western blot - Anti-SPOCD1 antibody (ab122188)All lanes : Anti-SPOCD1 antibody (ab122188) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
