Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-sPLA2-X antibody [EPR11202] (ab166634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11202] to sPLA2-X
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-sPLA2-X antibody [EPR11202]
    See all sPLA2-X primary antibodies

  • Description

    Rabbit monoclonal [EPR11202] to sPLA2-X

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human sPLA2-X.
    Database link: O15496

  • Positive control

    • TT, A549, MCF7 and Caco 2 cell lysates; Human pancreas and tonsil tissues; permeabilized A549 cells.

  • General notes

     This product was previously labelled as Phospholipase A2 X

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11202

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-sPLA2-X antibody [EPR11202] (ab166634)
    Western blot - Anti-sPLA2-X antibody [EPR11202] (ab166634)
    All lanes : Anti-sPLA2-X antibody [EPR11202] (ab166634) at 1/1000 dilution

    Lane 1 : TT cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling sPLA2-X with ab166634 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling sPLA2-X with ab166634 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-sPLA2-X antibody [EPR11202] (ab166634)
    Flow Cytometry - Anti-sPLA2-X antibody [EPR11202] (ab166634)
    Flow cytometric analysis of permeabilized A549 cells using ab166634 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
  • Anti-sPLA2-X antibody [EPR11202] (ab166634)
    Anti-sPLA2-X antibody [EPR11202] (ab166634)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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