Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)

Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6394] to SPAK - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Product image
Anti-ULK4 antibody (ab230041)
Product image
Recombinant Human IRE1 protein (ab114174)
Product image
Anti-LOK antibody (ab70483)
Product image
Anti-ULK3 antibody [EPR4888] (ab124947)

Overview

  • Product name

    Anti-SPAK antibody [EPR6394] - BSA and Azide free
    See all SPAK primary antibodies
  • Description

    Rabbit monoclonal [EPR6394] to SPAK - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248204 is the carrier-free version of ab128894.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 3.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6394
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Images

  • Western blot - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    Western blot - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)

    This data was developed using ab128894, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: SPAK knockout HAP1 whole cell lysate (20 µg)

    Lane 3: Jurkat whole cell lysate (20 µg)

    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab128894 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab128894 was shown to specifically react with SPAK in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SPAK knockout samples were uexamined. Wild-type and SPAK knockout samples were subjected to SDS-PAGE. ab128894 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    Western blot - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    All lanes : Anti-SPAK antibody [EPR6394] (ab128894) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : BxPC-3 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?



    This data was developed using ab128894, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    This data was developed using ab128894, the same antibody clone in a different buffer formulation.ab128894, at 1/50 dilution, staining SPAK in paraffin embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    OI-RD Scanning - Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    This data was developed using ab128894, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)
    Anti-SPAK antibody [EPR6394] - BSA and Azide free (ab248204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (ab172638)

  •  
  • Product image

    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

  •  
  • Product image

    Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

  •  
  • Product image

    Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

  •  
  • Product image

    FITC Anti-CD3 zeta antibody [H146-968] (ab91493)

  •  
  • Product image

    HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.