Anti-Sp8 antibody (ab73494)
Key features and details
- Rabbit polyclonal to Sp8
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Sp8 antibody -
Description
Rabbit polyclonal to Sp8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Sp8 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90836) -
Positive control
- This antibody gave a positive signal in 46C whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).Target
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Function
Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 221833 Human
- Entrez Gene: 320145 Mouse
- Omim: 608306 Human
- SwissProt: Q8IXZ3 Human
- SwissProt: Q8BMJ8 Mouse
- Unigene: 195922 Human
- Unigene: 51682 Mouse
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Alternative names
- BTD antibody
- Buttonhead antibody
- Homolog of Btd antibody
see all
Images
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Western blot - Anti-Sp8 antibody (ab73494)Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab73494 detects a band at 46 kDa in 46c whole cell lysate. We are unsure as to why the band seen is slightly lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.
Datasheets and documents
References (1)
ab73494 has been referenced in 1 publication.
- Borello U et al. SP8 Transcriptional Regulation of Cyclin D1 During Mouse Early Corticogenesis. Front Neurosci 12:119 (2018). ChIP-sequencing ; Mouse . PubMed: 29599703
Images
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Western blot - Anti-Sp8 antibody (ab73494)Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab73494 detects a band at 46 kDa in 46c whole cell lysate. We are unsure as to why the band seen is slightly lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.
