Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
![Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)](https://www.abcam.com/ps/products/250/ab250329/Images/ab250329-5-anti-sp17-antibody-epr6497-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6497(2)] to SP17 - BSA and Azide free
- Suitable for: IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free
See all SP17 primary antibodies -
Description
Rabbit monoclonal [EPR6497(2)] to SP17 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250329 is the carrier-free version of ab181079. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250329 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6497(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
This data was developed using ab181079, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SP17 with purified ab181079 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)](https://www.abcam.com/ps/products/250/ab250329/Images/ab250329-3-anti-sp17-antibody-epr6497-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329) -
![Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)](https://www.abcam.com/ps/products/250/ab250329/Images/ab250329-2-anti-sp17-antibody-epr6497-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)This data was developed using ab181079, the same antibody clone in a different buffer formulation.ab181079 (purified) at 1/90 immunoprecipitating SP17 in human testis tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST. -
![Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)](https://www.abcam.com/ps/products/250/ab250329/Images/ab250329-1-anti-sp17-antibody-epr6497-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)This data was developed using ab181079, the same antibody clone in a different buffer formulation.Western blot analysis of SP17 in immunoprecipitation pellets from Human testis tissue lysate. Unpurified ab181079 was used at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500. Lane 2 shows negative control. -
![Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)](https://www.abcam.com/ps/products/250/ab250329/Images/ab250329-4-benefits-of-recombinant-antibodies.png)
Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
