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Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SOX9 antibody [EPR12755-50] - C-terminal
    See all SOX9 primary antibodies

  • Description

    Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, SW480 and Caco2 cell lysates; Caco2 and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
    Western blot - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
    All lanes : Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Caco2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed Caco2 cells, labeling SOX9 with ab184547 at 1/500 dilution (red). Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells, labeling SOX9 with ab184547 at 1/500 dilution (green). Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Flow Cytometry - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
    Flow Cytometry - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

    Flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab184547 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
    Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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