Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23041-60] to SOX1 - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free
    See all SOX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23041-60] to SOX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebellum and hippocampus tissue; rat cerebellum tissue. IHC-Fr: Mouse and rat cerebellum tissue.

  • General notes

    ab263870 is the carrier-free version of ab242125. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the stem cells in rat cerebellum is observed. The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242125).

  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling SOX1 with ab242125 at 1/100 followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on rat cerebellum (PMID: 24676388) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242125).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Negative control: No staining on the mouse testis.

    The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242125).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the stem cells in mouse hippocampus is observed (PMID: 22992951). The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242125).

  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling SOX1 with ab242125 at 1/100 followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on mouse cerebellum (PMID: 24676388) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242125).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the stem cells in mouse cerebellum is observed (PMID: 24676388). The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242125).

  • Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
    Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

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