Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)
Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies -
Description
Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1. (Derived from C-terminal cytoplasmic region).
Database link: P19634 -
Positive control
- IHC-P: Human kidney tissue. ICC/IF: HEK-293T cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1. WB: Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate. Flow Cyt: Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cells.
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General notes
Previously labelled as Sodium / Hydrogen Exchanger 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)All lanes : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Myc-tagged Sodium/Hydrogen Exchanger 1/NHE-1 transfected HEK-293T cell lysate
Predicted band size: 91 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)Formalin-fixed, paraffin-embedded human-kidney tissue stained for Sodium/Hydrogen Exchanger 1/NHE-1 using ab230449 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transiently expressing Sodium/Hydrogen Exchanger 1/NHE-1 stained for Sodium/Hydrogen Exchanger 1/NHE-1 (red) using ab230449 at 1/100 dilution in ICC/IF. Left panel stained with Azami-green.
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Flow Cytometry - Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab230449)Flow cytometric analysis of intracellular Sodium/Hydrogen Exchanger 1/NHE-1 expression in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transeintly expressing Sodium/Hydrogen Exchanger 1/NHE-1 and Azami green, using Normal rabbit IgG (left) and ab230449 at 1/100 dilution (right).
