Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16762] to SNRPD2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free
    See all SNRPD2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16762] to SNRPD2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251254 is the carrier-free version of ab198296 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251254 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Sm-D2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Western blot - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    All lanes : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 30 seconds


    This data was developed using ab198296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)

    This data was developed using ab198296, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin. Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Western blot - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Lanes 1-6 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/2000 dilution
    Lane 7 : Anti-SNRPD2 antibody [EPR16762] (ab198296) at 1/20000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW 264.7 cell lysate
    Lane 7 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 30 seconds


    This data was developed using ab198296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    This data was developed using ab198296, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin. Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)

    This data was developed using ab198296&

    44; the same antibody clone in a different buffer formulation.

    >NRPD2 protein was immunoprecipitation from 1mg of MCF-7 (Human breast adenocarcinoma) whole cell lysate with ab198296 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198296 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, MCF-7 (Human breast adenocarcinoma) whole cell lysate, 10ug. Lane 2: IP of NRPD2 from MCF-7 (Human breast adenocarcinoma) whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198296 in MCF-7 (Human breast adenocarcinoma) whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Flow Cytometry - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    This data was developed using ab198296, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling SNRPD2 (red) with purified ab198296 at dilution of 1/150. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)
    Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (ab251254)

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