Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18718] to SLP-2 - BSA and Azide free
Suitable for: Flow Cyt, ICC, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-SLP-2 antibody [EPR18718] - BSA and Azide free
See all SLP-2 primary antibodies
Description

Rabbit monoclonal [EPR18718] to SLP-2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, ICC, IP, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251097 is the carrier-free version of ab191883. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251097 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR18718
Isotype

IgG
Research areas

Western blot - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 39 kDa

Exposure time: 1 minute

This data was developed using ab191883, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

All lanes : Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 30 seconds

This data was developed using ab191883, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

All lanes : Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : Rat brain lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

This data was developed using ab191883, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-4: 3 minutes; Lanes 5-6: 1 minute.
Immunocytochemistry - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

This data was developed using ab191883, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with ab191883 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor^®488 goat anti-rabbit (ab150077), used at a dilution of 1 in 1000 (shown in green). The mitochondria were stained with ab33985 (mouse monoclonal to COX-IV, mitochondrial marker) at 1/1000 and ab150120 at 1/1000 (shown in red). The cells were fixed in 100% methanol and permeabilized using 0.1% triton-X 100. The negative controls are shown in bottom panels. For negative control 1, ab191883 was used at a dilution of 1/250 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000. For negative control 2, ab7291 (mouse monoclonal to alpha tubulin) was used at a dilution of 1/1000 followed by ab150077 (Alexa Fluor^®488 goat anti-rabbit) at a dilution of 1/1000.
Immunocytochemistry - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

This data was developed using ab191883, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SLP-2 with ab191883 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab191883 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Immunocytochemistry - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

This data was developed using ab191883, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat cells (Human T cell leukemia cell line from peripheral blood) labeling SLP-2 with ab191883 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab191883 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Flow Cytometry - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

This data was developed using ab191883, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SLP-2 with ab191883 at 1/120 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

This data was developed using ab191883, the same antibody clone in a different buffer formulation.SLP-2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab191883 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab191883 at 1/1000 dilution. Veriblot for IP (HRP) (ab13136) was used as secondary antibody at 1/10000 dilution.
Lane 1: Jurkat whole cell lysate 10µg (Input).
Lane 2: ab191883 IP in Jurkat whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] (ab172730) instead of ab191883 in Jurkat whole cell cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Anti-SLP-2 antibody [EPR18718] - BSA and Azide free (ab251097)

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