Anti-Sld2 antibody (ab139172)
Key features and details
- Rabbit polyclonal to Sld2
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Sld2 antibody -
Description
Rabbit polyclonal to Sld2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Synthetic peptide corresponding to Saccharomyces cerevisiae Sld2 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P34252 -
Positive control
- This antibody gave a positive signal in both Exponentially Growing WT Yeast and W303 S.cerevisiae lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Saccharomyces cerevisiaeApplication Abreviews Notes WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.Notes WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.Target
- Information by UniProt
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Database links
- Entrez Gene: 853752 Saccharomyces cerevisiae
- SwissProt: P34252 Saccharomyces cerevisiae
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Alternative names
- DNA replication and checkpoint protein 1 antibody
- DNA replication regulator SLD2 antibody
- SLD2 antibody
- SLD2_YEAST antibody
Images
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Western blot - Anti-Sld2 antibody (ab139172)All lanes : Anti-Sld2 antibody (ab139172) at 1 µg/ml
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : W303 Yeast Lysate Whole Cell Lysate - Negative Control
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Additional bands at: 29 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 67 kDa (possible non-specific binding)
Exposure time: 30 secondsThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139172 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab139172 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Sld2 antibody (ab139172)All lanes : Anti-Sld2 antibody (ab139172) at 1 µg/ml
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : W303 Yeast Lysate Whole Cell Lysate - Negative Control
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Additional bands at: 29 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 67 kDa (possible non-specific binding)
Exposure time: 30 secondsThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139172 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
