Anti-SLC34A2 antibody [SP322] - N-terminal (ab228474)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP322] to SLC34A2 - N-terminal
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SLC34A2 antibody [SP322] - N-terminal
See all SLC34A2 primary antibodies -
Description
Rabbit monoclonal [SP322] to SLC34A2 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung adenocarcinoma, ovarian adenocarcinoma, endometrial adenocarcinoma, fallopian tube and uterus tissues. ICC/IF: NIH:OVCAR-3 cells Flow Cyt: NIH:OVCAR-3 cells
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP322 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the luminal surface of human endometrial carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228474 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228474 for 10 mins at room temperature. -
Immunocytochemistry/ Immunofluorescence analysis of NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labeling SLC34A2 with purified ab228474 at 1/100 (2.4 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of OvCAR-3 (human ovary adenocarcinoma) labeling SLC34A2 with purified ab228474 at 1/20 dilution (11.85 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
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Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.
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