Anti-SKAP55 antibody [EPR11360] (ab170882)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11360] to SKAP55
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-SKAP55 antibody [EPR11360]
See all SKAP55 primary antibodies -
Description
Rabbit monoclonal [EPR11360] to SKAP55 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SKAP55 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q86WV1 -
Positive control
- Human thymus, Jurkat, HuT-78 and MOLT4 lysates, Jurkat cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11360 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-SKAP55 antibody [EPR11360] (ab170882) at 1/1000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Jurkat lysate
Lane 3 : HuT-78 lysate
Lane 4 : MOLT4 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 41 kDa
-
Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).
-
Flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55 using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).
-