Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-SIRT7 antibody [EPR23811-15] (ab259968)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23811-15] to SIRT7
Suitable for: IP, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-SIRT7 antibody [EPR23811-15]
See all SIRT7 primary antibodies
Description

Rabbit monoclonal [EPR23811-15] to SIRT7
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Hepa1-6, Neuro-2a, NIH/3T3, RAW 264.7, C6, HeLa, HEK-293T, HepG2 and MCF7 whole cell lysates; Mouse spleen, skeletal muscle and liver tissue lysates; Ratskeletal muscle and liver tissue lysates. IHC-P: Mouse liver tissue; Rat spleen tissue. IP: HeLa and Neuro-2a whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23811-15
Isotype

IgG
Research areas

Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse liver (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

SIRT7 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug

Lane 2: ab259968 IP in Neuro-2a whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in Neuro-2a whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 67 seconds.
Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : Rat liver tissue lysate
Lane 5 : Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Low expression: skeletal muscle (PMID: 16618798).
Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

Lane 1 : Mouse spleen tissue lysate
Lane 2 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 6 : C6 (rat glial tumor glial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

The expression pattern of SIRT7 is consistent with what has been described in the literature(PMID: 24771643, 16618798).
Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

SIRT7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab259968 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat spleen (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Anti-SIRT7 antibody [EPR23811-15] (ab259968)

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