Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [SADF10.1] to SIRP alpha - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free
    See all SIRP alpha primary antibodies

  • Description

    Mouse monoclonal [SADF10.1] to SIRP alpha - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate. U937, SK-MEL-2 and SK-MEL-28 whole cell lysate. ICC/IF: THP-1 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab267410 is the carrier-free version of ab267409. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab267410 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)
    Western blot - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)
    All lanes : Anti-SIRP alpha antibody [SADF10.1] (ab267409) at 1.273 µg/ml

    Lanes 1 & 3 : Human brain tissue lysate
    Lane 2 : U937 (human histiocytic lymphoma monocyte), whole cell lysate
    Lane 4 : SK-MEL-2 (human malignant melanoma), whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 55 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile weight observed is consistent with what has been described in the literature (PMID: 9712903, 12773380).
    Negative control: Jurkat (PMID: 17982459).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267409).

  • Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)
    Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling SIRP alpha with ab267409 at 127.3ug/ml, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
    Negative control 1: ab267409 at 127.3µg/ml followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267409).

  • Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)
    Anti-SIRP alpha antibody [SADF10.1] - BSA and Azide free (ab267410)

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