Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRP alpha antibody [SADF10.1] (ab267409)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [SADF10.1] to SIRP alpha
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SIRP alpha antibody [SADF10.1]
    See all SIRP alpha primary antibodies

  • Description

    Mouse monoclonal [SADF10.1] to SIRP alpha

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate. U937, SK-MEL-2 and SK-MEL-28 whole cell lysate. ICC/IF: THP-1 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SIRP alpha antibody [SADF10.1] (ab267409)
    Western blot - Anti-SIRP alpha antibody [SADF10.1] (ab267409)
    All lanes : Anti-SIRP alpha antibody [SADF10.1] (ab267409) at 1.273 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : U937 (human histiocytic lymphoma monocyte), whole cell lysate
    Lane 3 : SK-MEL-28 (human malignant melanoma), whole cell lysate
    Lane 4 : SK-MEL-2 (human malignant melanoma), whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 55 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile weight observed is consistent with what has been described in the literature (PMID: 9712903, 12773380).
    Negative control: Jurkat (PMID: 17982459).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] (ab267409)
    Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [SADF10.1] (ab267409)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling SIRP alpha with ab267409 at 127.3ug/ml, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
    Negative control 1: ab267409 at 127.3µg/ml followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

     

  • Anti-SIRP alpha antibody [SADF10.1] (ab267409)
    Anti-SIRP alpha antibody [SADF10.1] (ab267409)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.