Anti-SIN1 antibody (ab277805)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [2HCLC] to SIN1
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SIN1 antibody [2HCLC]
See all SIN1 primary antibodies -
Description
Rabbit recombinant multiclonal [2HCLC] to SIN1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SIN1 aa 372-493.
Database link: Q9BPZ7 -
Positive control
- WB: HepG2, U87 MG, MCF7, T-47D and K562 whole cell extracts. Mouse and rat lung tissue extracts. ICC: MCF7 cells.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
2HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIN1 antibody (ab277805)
Western blot analysis was performed on whole cell extracts (30 μg lysate) of HepG2 (human liver hepatocellular carcinoma cell line) (Lane 1), U-87 MG (human glioblastoma-astrocytoma epithelial cell line) (Lane 2), MCF7 (human breast adenocarcinoma cell line) (Lane 3), T-47D (human ductal breast epithelial tumor cell line) (Lane 4), K562(human chronic myelogenous leukemia lymphoblast cell line) (Lane 5) and tissue extracts of mouse lung (Lane 6) and rat lung (Lane 7).
The blots were probed with ab277805 at 1 μg/mL.
The blots were detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L)-HRP secondary antibody, (0.4 μg/mL, 1/2500 dilution).
10% Bis-Tris gel.
The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed.
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Immunocytochemistry - Anti-SIN1 antibody (ab277805)For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous SIN1 using ab277805 at 2 µg/mL) and labeled with a Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate (1/2000).
Panel a) Shows representative cells that were stained for detection and localization of SIN1 protein (green).
Panel b) Stained for nuclei (blue) with DAPI.
Panel c) Cytoskeletal F-actin staining using Rhodamine Phalloidin (1/300).
Panel d) Composite image of panels a, b and c.
Panel e) Control cells with no primary antibody to assess background.
The images were captured at 60X magnification.
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Anti-SIN1 antibody (ab277805)