Anti-Signal peptide peptidase-like 2B antibody (ab43856)
Key features and details
- Rabbit polyclonal to Signal peptide peptidase-like 2B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Signal peptide peptidase-like 2B antibody -
Description
Rabbit polyclonal to Signal peptide peptidase-like 2B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Signal peptide peptidase-like 2B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab43855) -
Positive control
- This antibody gave a positive signal in WI38 Whole Cell Lysate, Human Thymus Tissue Lysate and Human Brain Tissue Lysate (data not shown).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab43856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).Target
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Relevance
The SPPLs are a group of aspartic proteases that are responsible for proteolysis of type II membrane-anchored signalling factors. SPPL2b, unlike other SPPL family members is found in endosomes/lysosomes, and not the endoplasmic reticulum, and has a role in catalysing intramembrane cleavage of tumour necrosis factor alpha (TNFalpha). -
Cellular localization
Membrane; multi-pass membrane protein of endosomes/lysosomes. -
Database links
- Entrez Gene: 56928 Human
- Omim: 608239 Human
- SwissProt: Q8TCT7 Human
- Unigene: 744026 Human
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Alternative names
- IMP4 antibody
- Intramembrane protease 4 antibody
- Protein SPP-like 2B antibody
- SPPL2B antibody
Images
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Western blot - Anti-Signal peptide peptidase-like 2B antibody (ab43856)All lanes : Anti-Signal peptide peptidase-like 2B antibody (ab43856) at 1 µg/ml
Lane 1 :WI-38 whole cell lysate (ab3960)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4) of Signal peptide peptidase-like 2B. The predicted molecular weights of isoforms (1,2,3,4) are 65 kDa, 35 kDa, 34 kDa and 56 kDa respectively. The band observed at 37 kDa may represent isoform 2 of Signal peptide peptidase-like 2B.
Datasheets and documents
References (0)
ab43856 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Signal peptide peptidase-like 2B antibody (ab43856)All lanes : Anti-Signal peptide peptidase-like 2B antibody (ab43856) at 1 µg/ml
Lane 1 :WI-38 whole cell lysate (ab3960)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4) of Signal peptide peptidase-like 2B. The predicted molecular weights of isoforms (1,2,3,4) are 65 kDa, 35 kDa, 34 kDa and 56 kDa respectively. The band observed at 37 kDa may represent isoform 2 of Signal peptide peptidase-like 2B.
