Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22438-93] to SIGIRR - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free
    See all SIGIRR primary antibodies

  • Description

    Rabbit monoclonal [EPR22438-93] to SIGIRR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney and colon tissue; Mouse kidney tissue; Rat kidney tissue WB: Hepa1-6 cell lysate; Mouse stomach, kidney, spleen and pancreas tissue lysate; Human stomach and fetal kidney lysate; Rat stomach and liver tissue lysate Flow cyt: MEF and Hepa1-6 cells IP: Hepa1-6 whole cell lysate

  • General notes

    Ab256314 is the carrier-free version of ab228977. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256314 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SIGIRR with ab228977 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney (PMID:14715412, 12925853). The section was incubated with ab228977 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228977).

  • Flow Cytometry - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Flow Cytometry - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MEF (mouse embryonic fibroblast (immortalized)) (Left) / Hepa1-6 (mouse hepatoma epithelial cell line) (Right) cells labelling SIGIRR with ab228977 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: MEF (PMID 12925853).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228977).

  • Immunoprecipitation - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Immunoprecipitation - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

    IGFBP5 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate 10μg with ab254324 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab228977 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate 10μg

    Lane 2: ab254324 IP in Hepa1-6 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228977 in Hepa1-6 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228977).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling SIGIRR with ab228977 at 1/1000 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID:14715412, 12925853). The section was incubated with ab228977 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228977).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SIGIRR with ab228977 at 1/1000 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID:21077278) The section was incubated with ab228977 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228977).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SIGIRR with ab228977 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney (PMID:14715412, 12925853) The section was incubated with ab228977 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228977).

  • Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)
    Anti-SIGIRR antibody [EPR22438-93] - BSA and Azide free (ab256314)

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