Anti-SHP1 (phospho S591) antibody (ab41436)
Key features and details
- Rabbit polyclonal to SHP1 (phospho S591)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHP1 (phospho S591) antibody
See all SHP1 primary antibodies -
Description
Rabbit polyclonal to SHP1 (phospho S591) -
Host species
Rabbit -
Specificity
Phospho-Ser591-SHP1 specific. This antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho-Ser591-SHP1 peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to a non specific phospho-serine peptide then affinity purified using phospho-Ser591-SHP1 peptide (immunogen). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHP1 (phospho S591) antibody (ab41436)Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-6 : Anti-SHP1 (phospho S591) antibody (ab41436) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Ser591-SHP1 peptide
Lane 6 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with non specific phospho-serine peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
