Anti-ANP32E antibody (ab5993)
Key features and details
- Rabbit polyclonal to ANP32E
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ANP32E antibody
See all ANP32E primary antibodies -
Description
Rabbit polyclonal to ANP32E -
Host species
Rabbit -
Specificity
This antibody does not cross-react with PHAPI or PHAP12a. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ANP32E (C terminal).
(Peptide available asab6242) -
Positive control
- A549 or HepG2 whole cell lysate (ab7900).
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General notes
This product was previously labelled as LANPL
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ANP32E antibody (ab5993)
Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with anti-PHAP III at 1 µg/ml. Western blot analysis of ANP32E expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with ab5993 at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANP32E antibody (ab5993)ab5993 at 2µg/ml staining ANP32E in human small intestine tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-ANP32E antibody (ab5993)ICC/IF image of ab5993 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5993, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-ANP32E antibody (ab5993)Immunofluorescence of PHAP III in Human Small Intestine cells using ab5993 at 20 ug/ml.
