Anti-SHANK2 antibody [S23b-6] (ab242005)
![Anti-SHANK2 antibody [S23b-6] (ab242005)](https://www.abcam.com/ps/products/242/ab242005/Images/ab242005-332397-anti-shank2-antibody-s23b-6-immunofluorescence.jpg "Anti-SHANK2 antibody [S23b-6] (ab242005)")
Key features and details
- Mouse monoclonal [S23b-6] to SHANK2
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SHANK2 antibody [S23b-6]
See all SHANK2 primary antibodies -
Description
Mouse monoclonal [S23b-6] to SHANK2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Rat SHANK2 aa 50-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (SH3/PDZ domains) NP_958738.1
Database link: Q9QX74 -
Positive control
- ICC/IF: SK-N-BE cells. WB: Rat brain membrane lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
S23b-6 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [S23b-6] (ab242005)
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling SHANK2 using ab242005 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min RT, 5 min RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab242005 (D) Composite.
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![Western blot - Anti-SHANK2 antibody [S23b-6] (ab242005)](https://www.abcam.com/ps/products/242/ab242005/Images/ab242005-332396-anti-shank2-antibody-s23b-6-western-blot.jpg)
Western blot - Anti-SHANK2 antibody [S23b-6] (ab242005)Anti-SHANK2 antibody [S23b-6] (ab242005) at 1/1000 dilution (2 hours at RT) + Rat brain membrane lysate at 15 µg
Secondary
Sheep anti-Mouse IgG-HRP,1 hour at RT
Predicted band size: 159 kDaBlock: 1.5% BSA for 30 minutes at RT.
