Anti-SH2D1A/SAP antibody [1D12] (ab252866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1D12] to SH2D1A/SAP
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-SH2D1A/SAP antibody [1D12]
See all SH2D1A/SAP primary antibodies -
Description
Rat monoclonal [1D12] to SH2D1A/SAP -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- WB: MOLT-4, Jurkat and HDLM-2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1D12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-SH2D1A/SAP antibody [1D12] (ab252866) at 1/1000 dilution
Lane 1 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : HDLM-2 (human Hodgkin lymphoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution
Lanes 2-4 : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 14 kDa
Observed band size: 14,40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Raji (PMID:11313386; PMID:11054674).
Exposure time: lane 1, 2:10 seconds lane 3, 4: 37 seconds
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