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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-SH2D1A/SAP antibody [1D12] (ab252866)

Price and availability

307 566 ₸

Availability

Order now and get it on Tuesday March 23, 2021

Anti-SH2D1A/SAP antibody [1D12] (ab252866)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1D12] to SH2D1A/SAP
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-SH2D1A/SAP antibody [1D12]
    See all SH2D1A/SAP primary antibodies
  • Description

    Rat monoclonal [1D12] to SH2D1A/SAP
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: MOLT-4, Jurkat and HDLM-2 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1D12
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling

Images

  • Western blot - Anti-SH2D1A/SAP antibody [1D12] (ab252866)
    Western blot - Anti-SH2D1A/SAP antibody [1D12] (ab252866)
    All lanes : Anti-SH2D1A/SAP antibody [1D12] (ab252866) at 1/1000 dilution

    Lane 1 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : HDLM-2 (human Hodgkin lymphoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution
    Lanes 2-4 : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14,40 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Raji (PMID:11313386; PMID:11054674). 

    Exposure time: lane 1, 2:10 seconds lane 3, 4: 37 seconds

  • Anti-SH2D1A/SAP antibody [1D12] (ab252866)
    Anti-SH2D1A/SAP antibody [1D12] (ab252866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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