Anti-SH2B1/PSM antibody (ab228828)
Key features and details
- Rabbit polyclonal to SH2B1/PSM
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SH2B1/PSM antibody
See all SH2B1/PSM primary antibodies -
Description
Rabbit polyclonal to SH2B1/PSM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment within Human SH2B1/PSM (internal sequence). The exact sequence is proprietary.
Database link: Q9NRF2 -
Positive control
- WB: C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat brain tissue extracts. ICC/IF: A431 cells.
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General notes
This product was previously labelled as SH2B1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SH2B1/PSM antibody (ab228828) at 1/2000 dilution
Lane 1 : Non-transfected C2C12 (mouse myoblast cell line) whole cell extract
Lane 2 : C2C12 (mouse myoblast cell line) transfected with SH2B1/PSM shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa10% SDS-PAGE gel.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for SH2B1/PSM (green) using ab228828 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-Alpha-tubulin antibody at 1/2000 dilution (red).
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All lanes : Anti-SH2B1/PSM antibody (ab228828) at 1/2000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-SH2B1/PSM antibody (ab228828) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa10% SDS-PAGE gel.