Anti-SETD5 antibody (ab204363)
Key features and details
- Rabbit polyclonal to SETD5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SETD5 antibody -
Description
Rabbit polyclonal to SETD5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human SETD5 aa 550-650.
Database link: Q9C0A6 -
Positive control
- WB: Transfected Rh-30 whole cell lysates; IHC-P: Human endometrium, cerebral cortex, skin and kidney tissue; ICC/IF: U-2 OS whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-SETD5 antibody (ab204363)All lanes : Anti-SETD5 antibody (ab204363) at 0.4 µg/ml
Lane 1 : RH-30 (human bone marrow metastasis cell line) with control siRNA, cell lysate
Lane 2 : RH-30 with target specifc siRNA probe, cell lysate
Predicted band size: 158 kDaLoading control: Anti-GAPDH
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD5 antibody (ab204363)Immunohistochemical analysis of human endometrium tissue labeling SETD5 in the nucleus of glandular cells with ab204363 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-SETD5 antibody (ab204363)Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling SETD5 in the nucleoplasm (Green) with ab204363 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD5 antibody (ab204363)Immunohistochemical analysis of human kidney tissue labeling SETD5 in the nucleus of cells tubules with ab204363 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD5 antibody (ab204363)Immunohistochemical analysis of human skin tissue labeling SETD5 in the nucleus of squamous epithelial cells with ab204363 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SETD5 antibody (ab204363)Immunohistochemical analysis of human cerebral cortex tissue labeling SETD5 in the nucleus of neurons with ab204363 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.